Thp-1 phagocytosis
THP-1 (TIB-202 from ATCC) cells were cultured in RPMI1640 + Glutamax-I Medium (Gibco, 61870-010) with 10% FCS (Invitrogen, 50064), 1% sodium pyruvate (Gibco, 11360-070) and 1% penicillin/streptomycin (Gibco, 15140-122) at 37 °C and 5% CO2. Cells were passaged twice per week and kept at a cell … See more Design, construction, and basic performance of the genome-wide sgRNA library has been described in Michlits et al.21. To generate focused sgRNA pools for … See more Lentiviral vectors were produced using MISSION Lentiviral Packaging Mix (Sigma-Aldrich), PEI Max (Polysciences, Inc.) and Lenti X-293T cells (Clontech … See more THP-1 iCas9 cells were transduced with lentiviral particles using spinfection. To this end, THP-1 cells were transferred into 6-well plates, virus was added and … See more FACS-enriched or baseline cells were collected by centrifugation, washed with PBS and a maximum of 5E6 cells were resuspended in 350 µL DNA extraction … See more
Thp-1 phagocytosis
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WebTherefore, we established a macrophage polarization model using GBM cell lines and THP-1 monocyte cells. In this model, human THP-1 monocytes were first differentiated into M0 macrophages by incubation with phorbol 12-myristate 13-acetate (PMA) until the CD11b marker was upregulated (Figure 2A, Supplementary Figure S2A). WebAbstract. OBJECTIVE: To investigate the effect of serum amyloid A1 (SAA1) on global gene expression in macrophages derived from THP-1 monocytes. MATERIALS AND METHODS: Global genetic expression in THP-1-derived macrophages was determined using Illumina HT-12 microarray chips and the results were validated by real-time PCR.
WebJan 10, 2024 · Our detailed analyses of cytoskeletal filaments suggest a phagocytosis control system involving Ca2+ influx, the destabilization of ... DYNC1I2, DYNLL1, and DYNLT1. As the commonly expressed dynein complex was used in the THP-1-derived macrophage and its moving speed was not accelerated, other events, such as lysosome ... WebMar 14, 2024 · THP-1 cells differentiated with phorbol 12-myristate 13-acetate (PMA) are widely used as a model for function and biology of human macrophages. However, the …
Web1 day ago · FcγR expression of THP-1-CD16A macrophages was 168 evaluated by flow cytometry using fluorescent antibodies to FcγRI (clone 10.1), FcγRIIA 169 (clone IV.3), FcγRIIA/B/C (clone AT10), and FcγRIIIA/B (clone 3G8). 170 In vitro macrophage phagocytosis of ITP serum-opsonized platelets 171 Human macrophage phagocytosis … WebHowever, Hela cells might represent a different mechanism of USP8-mediated reduction of intracellular bacteria compared to THP-1 and RAW 264.7 macrophage cell models since in epithelial cells, Salmonella invasion process depends on the bacterial secretory system proteins encoded within SPI-1 while the invasion of macrophages is controlled mainly by …
WebThe anti-VSA antibodies promote antibody-dependent cellular phagocytosis (ADCP) of IEs ... an efficient and high-throughput plate-based flow cytometric assay to measure ADCP of …
WebTHP-1. TIB-202 ™. THP-1 is a monocyte isolated from peripheral blood from an acute monocytic leukemia patient. This cell line can be used in immune system disorder … northern kentucky eye care dr schuckWebFeb 24, 2024 · THP-1 cells suspended at a concentration of 1 × 10 6 in 1 ml of culture medium were stained with the Latex Beads-rabbit IgG-FITC Complex from the kit for 20 ... either phagocytosis or lysosomal pH on THP-1 cells was evaluated after treatment with increasing concentrations of LPS ranging from 0.01 to 1 μg/ml to examine whether ... northern kentucky family law attorneysWebIn the present study, we analyze the phagocytosis of wasteosomes and the mechanisms involved in this process. Accordingly, we purified wasteosomes from post-mortem … how to root att samsung galaxy tab 2 1.1WebKelley, S.M.; Ravichandran, K.S. Putting the brakes on phagocytosis: “don’t-eat-me” signaling in physiology and disease. EMBO Rep. 2024, 22, e52564. ... THP-1 cells were pretreated … how to root asus zenpad s3 10WebJan 13, 2010 · Differentiated macrophages are the resident tissue phagocytes and sentinel cells of the innate immune response. The phenotype of mature tissue macrophages … how to root a thanksgiving cactusWebAug 1, 2024 · Our data suggest that THP-1 may be of use when performing studies involving M1 polarization and phagocytosis, rather than M2 polarization. In conclusion, THP-1 Mφ … northern kentucky family counselingWebTHP-1 is a human monocytic cell line derived from an acute monocytic leukemia patient. It is used to test leukemia cell lines in immunocytochemical analysis of protein-protein … northern kentucky furniture outlet stores