Thp-1 macrophage-like cells
WebInvivoGen offers several engineered THP-1 cell lines: THP1 NF-κB cells were specifically designed for monitoring the NF-κB signal transduction pathway in a physiologically relevant cell line. They feature either the SEAP or the Lucia luciferase reporter gene. THP1-Dual™ cells allow the simultaneous study of the NF-κB pathway, by monitoring ... WebJan 7, 2024 · We next assessed the functionality of NK-4-treated THP-1 macrophages by analyzing cytokine production. NK-4 dose-dependently increased THP-1 macrophage secretion of the proinflammatory cytokine TNF-α after 3 days of culture (Fig. 1b). At 4 μM NK-4, TNF-α secretion increased by 36-fold compared to control cells (Fig. 1b). NK-4 did …
Thp-1 macrophage-like cells
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http://lcgdbzz.org/en/article/doi/10.3969/j.issn.1001-5256.2024.04.027 WebJun 26, 2024 · Experiments were performed on human THP-1, a human monocyte-like cell line derived from a patient with leukaemia, obtained from ATCC bank (ATCC# ... Repnik U et al (2024) Co-culture of human alveolar epithelial (hAELVi) and macrophage (THP-1) cell lines. Altex 35:211–222. PubMed Google Scholar Latz E, Xiao TS , Stutz A (2013 ...
WebMar 24, 2024 · To differentiate U937 and THP-1 cells into active macrophage-like cells, the cells were resuspended in culture medium containing 0.1 μM 1α, 25-dihydroxyvitamin D 3 … WebRegulation of Notch 1 signaling in THP-1 cells enhances M2 macrophage differentiation[J]. Am J Physiol Heart Circ Physiol, 2014, 307(11): H1634-H1642. DOI: …
WebApr 4, 2024 · Following differentiation of the THP-1 cells, MERTK upregulation was confirmed by western blot and flow cytometry. In a similar fashion, treatment with of murine bone marrow derived monocytic ... Web2 days ago · Briefly, the monocyte-like cell line THP-1 was grown in RPMI1640 medium containing 10% fetal bovine serum at 37 ℃ with 5% CO 2. The THP-1 cells were then …
WebHere, macrophage‐like THP‐1 cells, exposed to ten different CNM for 48 h in low‐cytotoxic concentration of 10 µg mL−1, are characterized by secretion of ... epithelial type-II BEAS …
WebMar 2, 2024 · In vitro studies using human monocytic cell lines such as THP-1, U937, MonoMac 6, ML-2 and HL-60 are frequently used to study monocyte/macrophage differentiation and function . The THP-1 used in this study are highly plastic and sensitive to many stimuli, and therefore can be polarized into multiple lineages (for review). boyne chair liftWebJul 29, 2008 · Although monocytic leukemia cell lines such as THP-1, KG-1, K562, monomac-6, and U-937 had originally been predicted to be good candidates for differentiation into DC-like cells, these leukemia cell lines have been demonstrated to be poor models for DC differentiation, most likely as they are already developed too far along … boyne castle irelandWebThe invention discloses a construction method of a mononuclear macrophage model based on HIV-1 (human immunodeficiency virus type 1) mass infection duplication, which … gw2 spirit of the finite resultWebTHP-1 cells (human monocytic cell line) were cultured in 6-well cell culture plates (1×10 6 cells per well) and maintained in complete RPMI 1640 medium at 37°C. For differentiation to a macrophage phenotype, THP-1 cells were treated with 10 ng/ml phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich) for 72 h, followed by a recovery period of 24 h in complete … gw2 spellbreaker wvw buildWebThe invention discloses a construction method of a mononuclear macrophage model based on HIV-1 (human immunodeficiency virus type 1) mass infection duplication, which comprises the following steps: designing a pair of identification sequences for SAMHD1 gene CDS1, and constructing a TALEN plasmid pair; transfecting the TALEN plasmid pair … boyne city boat storageWebThe severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease-19 (COVID-19). The spike protein (S) of SARS-CoV-2 plays a crucial role in mediating viral infectivity; hence, in an extensive effort to boyne city 4th of july 2022WebFigure 1 IL-17 induces M2-like phenotype in THP-1-derived macrophages. Notes: (A) THP-1-derived macrophages were treated with IL-17 at 0, 10, 50 and 100 ng/mL for 48 hours. The … boyne city apartments for rent