WebDec 1, 2004 · The results reported here have identified yueB as the essential gene involved in irreversible binding of bacteriophage SPP1 to Bacillus subtilis.First, a deletion in an SPP1-resistant (pha-2) strain, covering most of the yueB gene, could be complemented by a xylose-inducible copy of yueB inserted at amyE.Second, disruption of yueB by insertion … WebDownload scientific diagram Construction of a fusion B. subtilis mutant of ysxC . (A) Schematic representation of the ysxC region of BFA2414 after integration of pYSXCF, which is a pMUTIN4-based ...
Construction of a fusion B. subtilis mutant of ysxC . (A) Schematic ...
Web(pMUTIN4) Ap Em: MC1061(pMUTIN4) Shipping Conditions: LB agar with ampicillin (100 µg/ml) Growth Conditions: LB agar with ampicillin (100 µg/ml) Temperature: 37: … WebAug 1, 2005 · As the activity of the CssRS two‐component system determines the level of HtrA‐type proteases in the cell envelope of B. subtilis (Hyyryläinen et al. 2001; Darmon et al. 2002), the cssR gene was mutated (cssR::pMUTIN4) and its effects on the secretion of the various heterologous proteins and growth properties of the strains were determined. pain in penis head
Secretion of heterologous proteins in Bacillus subtilis can be …
WebOct 21, 2024 · The pMUTIN4-GFP and pBBR1MCS2-TAC-mCherry plasmids bought from Wuhan Miaoling Biotechnology Co., Ltd. were electrotransformed into BvL03 and AhX040 using the method of Pérez and Kimoto (Perez-Ramos … WebJul 12, 2024 · Following inoculation the array plates were incubated at 3.Iv1023 Liv1024 Liv1027 Liv1028 Liv1090 Liv1091 Liv1097 Liv1098 Plasmids pLTV1 pMUTIN4 pDG1513 … WebMar 7, 2002 · The β-galactosidase activities of B. subtilis 168 htrB::pMutin4 (BFA3041) and the B. subtilis 168 cssS::Sp htrB::pMutin4 strain (BV2015) were measured as a function of growth in TY medium at 37°C. As shown in Fig. Fig.2A, 2A , the htrB transcription peaked at about 1 h before the transition ( t = 0) between exponential and postexponential growth. subharti university mba it