WebMay 1, 2024 · Dynamic mass redistribution assay. The DMR assay was performed using an Epic Benchtop System (Corning, New York, USA). The principle behind the method has been described elsewhere (Fang, Li, & Ferrie, 2007; Schröder et al., 2011). On the day of the DMR assay, cells were washed three times using an electronic Viaflo 384-channel … Webto dynamic mass redistribution [4] The theories of operation for optical biosensor-based affinity profiling have been extensively discussed [1-2]. However,
Applying label-free dynamic mass redistribution technology to …
WebThus, we used label-free dynamic mass redistribution (DMR) assays to pharmacologically identify endogenous a 1-AR sub-types functionally expressed in SW480 colon carcinoma cells, thereby facilitating their examination as potential antineo-plastic drug targets. Materials and Methods Reagents and Plasmids. Human a 1B-AR cDNAs were subcloned WebJun 10, 2024 · Dynamic mass redistribution (DMR) is a label-free technology that utilises an optical biosensor to measure the redistribution of cellular constituents upon receptor stimulation 27. The biosensor used for measuring DMR is a resonant waveguide grating (RWG), consisting of a substrate and a cover layer with an embedded grating structure, … shenandoah vineyards plymouth ca
Dynamic mass redistribution reveals diverging importance of PDZ …
WebFeb 17, 2024 · Ligand-induced dynamic mass redistribution (DMR) measurements were performed on a stable clone exclusively overexpressing the sweet receptor subunits, … WebApr 12, 2024 · Dynamic mass redistribution (DMR) assays. 50 values were calculated based on the amplitudes of DMR signals at 20 min post stimulation of dopamine. All studies were carried out in two independent measurements, each with at least three replicates. 2.11. Construction of molecular network. WebAug 3, 2011 · A Dynamic Mass Redistribution Assay for the Human Sweet Taste Receptor Uncovers G-Protein Dependent Biased Ligands 17 February 2024 Frontiers in … spot it cards pdf