WebCy3 dye. Invitrogen Cy3 dye is a bright, orange-fluorescent dye that can be excited using the 532 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. In addition to immunocytochemistry applications, Cy3 dye is commonly used to label nucleic acids. The Cy3 fluorophore is also the basis for the lipophilic neuronal and long ... http://www.ultrapurebio.com/news-detail.asp?id=635&pid=259
Cy5 多荧生物 - 最全的荧光方案
WebNov 1, 2024 · 此外,Cy3 和 Cy5 还可用于 FRET 试验。 青色素染料是一种相对较老的荧光染料,但却是其他荧光染料在亮度、耐光性、量子产率等方面得以的基础。 在吸收紫外线或可见光后,能把短波长的光转变为波长较长的可见光波而反射出来,呈闪亮的鲜艳色彩。 Web这些染料都可以通过反应基团与核酸或蛋白质相连。例如,蛋白质标记使用马来酰胺基团。有趣的是,荧光方面Cy5对周围的电子环境很敏感,这可用于酶的测定。黏附蛋白的构象变化导致荧光发射的正负变化。此外,Cy3和Cy5可用于FRET实验。 canada border duty limits
An Introduction to Fluorescence Resonance Energy …
WebThey are thus able to easily distinguish colors from Cy3 and from Cy5, and also able to quantify the amount of Cy3 and Cy5 labeling in one sample (multiparametric detection). Other cyanine dyes are useful: Cy3.5 can replace sulfoRhodamine 101. Cy5.5 is a near-infrared (IR) fluorescence-emitting dye (excitation/emission maximum 678/694 nm). WebCy3还可以和Cy5-起在共聚焦显微镜实验中作多标记。. Cy5耦联基团的激发波长最大650nm,发光波长最大670nm。在氪氩灯(647nm)下它们可被激发出98%的荧光,在氦氖 … WebFRET 程度与供、受体分子的空间距离紧密相关, 一般为7~10 nm 时即可发生FRET; 随着距离延长, FRET呈显著减弱。. 供体和受体之间FRET的效率,可以由E=1/1+(R/R0)exp6 … fished acronym