WebDec 23, 2024 · The determined structure and identified metal-ion-binding sites of the precatalytic DNAzyme–RNA complex reveal that the basis of the DNA-mediated … WebDec 1, 2024 · RNA-cleaving NAzymes have been widely utilized as biorecognition elements for binding of metal ions, RNA, and DNA targets. Metal ions are shown to be essential for the activity of RNA-cleaving NAzymes as they induce changes in the 3D folding and conformation of the NAzymes to trigger catalysis [ 13, 14 ].
DNAmoreDB, a database of DNAzymes - PMC - National Center …
WebARTICLE Crystal structure of an RNA-cleaving DNAzyme Hehua Liu1,2, Xiang Yu1,2, Yiqing Chen1, Jing Zhang1, Baixing Wu 2, Lina Zheng2, Phensinee Haruehanroengra3, … WebOct 14, 2024 · Since the discovery of the first DNAzyme, a lead-dependent RNA-cleaving deoxyribozyme , hundreds of deoxyribozymes have been isolated, ... Crystal structure … greenview house supported living
Crystal structure of an RNA-cleaving DNAzyme – DOAJ
WebOrganic & Biomolecular chemistry, Royal Society of chemistry 18 de mayo de 2024 The RNA-cleaving 8–17 DNAzyme, which is a metalloenzyme that depends on divalent metal ions for its function, is... WebDec 1, 2024 · Three crystal structures of the 8–17 DNAzyme that include the pre-catalytic state of the RNA cleavage reaction are reported, providing insight into the catalytic … WebThe present invention provides a method for producing single stranded DNA polynucleotides. In particular, the invention provides a method that utilises a DNA minicircle obtained from a parental minicircle plasmid as a template in an enzyme-mediated rolling circle amplification (RCA) reaction to generate a product that can be cleaved to provide … fnf online all weeks