WebCohesion definition, the act or state of cohering, uniting, or sticking together. See more. WebMar 22, 2024 · The cohesins bind tightly and specifically to dockerin-bearing cellulosomal subunits, which comprise a dockerin domain (Doc), one or more enzymatic domains, …
Cohesin-Dockerin Interactions and Folding - Wiley Online Library
Our work focuses on the molecular basis of the interaction between the binding domains of the cellulosome using biochemical and molecular biological techniques. We employed progressive deletion and alanine-scanning techniques on a representative type-I dockerin module from Clostridium … See more Cohesin modules are the major building blocks of scaffoldins which are responsible for organizing the cellulolytic subunits into the multi-enzyme … See more The dockerin module anchors the catalytic subunits to the scaffoldin. It displays internal two-fold symmetry, consisting of a duplicated F-hand motif (a calcium-binding loop preceding an … See more In this study, we characterized functionally and structurally a unique, atypical type-III dockerin from the Ruminococcus flavefaciens ScaA scaffoldin (ScaADoc). By using different spectroscopic measurements we … See more The cohesin-dockerin pair represents a high-affinity (Kd 10-9-10-12M) protein-protein interaction. This interaction tends to be nonspecific within a given species but specific between species. Thus, a scaffoldin-borne … See more WebApr 20, 2024 · To colocalizing multienzyme pathways on the LDs’ membrane, a protein-based scaffold was developed based on Ole and cellulosomic cohesion-dockerin interaction pairs which include, Ole and two dockerin-cohesin pairs [X82 (a cohesin-like protein) and dockerin from Clostridium perfringens, cohesin and dockrein from … stepper scooter for adults
Cellulosome - CAZypedia
WebJan 1, 2011 · Therefore, this dockerin was chosen as a ‘typical’ dockerin for a quantitative SPR analysis. As shown in Table 2, the wild-type dockerin, rGST-Xyn10C, had a high affinity for the cognate cohesin proteins, rCoh1-Ct and rCoh3-Ct, but not for the noncognate C. josui cohesin proteins. When the ‘ST’ motif in the first segment of Xyn10C ... WebDec 11, 2012 · A cohesin amino acid mutation (D39A) that eliminated hydrogen bonding with the dockerin's critically conserved serine residues reduced the observed rupture … WebJan 12, 2024 · Cellulosomal enzymes are bound to a non-enzymatic membrane-anchoring protein, a scaffoldin, via a modular dockerin domain attached to the enzymes which interact and bind with cohesion proteins on the scaffoldin [60, 61], thus creating dynamic and powerful catabolic complexes (see Fig. 2). pipe projecting from fill